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We had previously developed a simple, chromatography-free, green method for the synthesis of chromene derivatives 15 , and we evaluated the anti-mycobacterial activity of some chromenes 16

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160 2D LC-ICP-MS holds considerable promise for biologically required metals discussed here, potentially capable of detecting a change in metalation status as a function of growth conditions, for example, but appears to be readily applied to a quantitative analysis of only the most abundant protein-metal complexes of high thermodynamic (Cu I , Zn II ) or kinetic stability